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Thermo Fisher
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Image Search Results
Journal: Cellular & Molecular Biology Letters
Article Title: The O -glycosylating enzyme GALNT2 acts as an oncogenic driver in non-small cell lung cancer
doi: 10.1186/s11658-022-00378-w
Figure Lengend Snippet: miR-30d negatively regulates GALNT2 expression in NSCLC. A Four databases were employed to predict the potential miRNAs targeting GALNT2. B Heatmaps of the top 50 miRNAs negatively associated with GALNT2 in NSCLC. C Analysis of miR-30d expression in TCGA. D Analysis of miR-30d expression using cDNA microarrays. E Detection of GALNT2 expression by RT-qPCR. F Detection of GALNT2 expression by Western blot. G Schematic representation of the putative binding site for miR-30d in the GALNT2 3′-UTR. H Luciferase reporter assay. I CCK-8 assay. J Colony formation assay. K Transwell migration and Matrigel invasion assays (magnification, ×100). OV GALNT2 overexpression lentivirus. # P > 0.05, * P < 0.05
Article Snippet: For miRNA detection, RNA was reverse transcribed into cDNA by using the miRNA
Techniques: Expressing, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Reporter Assay, CCK-8 Assay, Colony Assay, Migration, Over Expression